molecular characterization of echinococcus granulosus from hydatid cysts isolated from human and animals in golestan province, north of iran
نویسندگان
چکیده
b ackground: the aim of the present study was to determine the molecular characteristics of echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using pcr- rflp of its1- rdna analysis in golestan province, northern iran. m ethods: e . granulosus isolates from human patients infected with hydatid cyst and protoscoleces from hydatid cysts of sheep, cattle and camel isolates were collected from different hospitals and the abattoir throughout the golestan province. in all, 60 e. granulosus genomic dna were extracted and examined by pcr - its1 of rdna and amplified using bd1 / 4s and egf1 / egr2 primers, fol- lowed by rflp using alu1, msp1 and taqi restriction enzymes. r esults: the pcr-its1 products obtained from sheep, cattle and human isolates were similar to sheep strain (1000 bp and 391 bp). majority of the camel samples yielded 295 bp dna bands. rflp-its1 of e. granulosus with taq1 in human, sheep and cattle isolates showed similar patterns in the number and size of dna. rflp methods in camel isolates showed a different genotype, using taq1 , whereas no dna bands were observed using alu1 in camel and human isolates. therefore, two clearly distinguishable banding patterns of e. granulosus were obtained with the three enzymes, which separating human, sheep and cattle isolates from the camel origin. c onclusion: the results indicate the possible of transmission of the g1 and g6 genotypes of e. granulosus between livestock animals and human in golestan province.
منابع مشابه
Molecular Characterization of Echinococcus granulosus from Hydatid Cysts Isolated from Human and Animals in Golestan Province, North of Iran
BACKGROUND The aim of the present study was to determine the molecular characteristics of Echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using PCR- RFLP of ITS1- rDNA analysis in Golestan Province, northern Iran. METHODS E. granulosus isolates from human patients infected with...
متن کاملMolecular Characterization of Echinococcus granulosus Isolated from Sheep and Camel in Iran
متن کامل
Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran.
Cystic echinococcosis is endemic in Iran, particularly in Ardabil Province, where it causes health and economic problems. The genetic pattern of Echinococcus granulosus has been determined in most parts of Iran, except in this area. In the present investigation, 55 larval isolates were collected from humans (11), sheep (19), goats (4) and cattle (21). For analysis of the genetic characteristics...
متن کاملMolecular Characterization of Echinococcus granulosus Isolated from Sheep and Camel in Iran
SUmmJlry Forty-eight isolates of hydatid cyst collected from sheep and camels slaughtered in different areas of Iran were analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. The results indicated that the isolates could be categorized into two distinct and uniform genotype grouping, the camel/dog strain,group (Genotype 6) and cosmopolitan common sheep ...
متن کاملIdentification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan – Iran
BACKGROUND The aim of this study was DNA extraction from protoscolecses of Echinococcus granulosus and identification of these strains in West-Azerbaijan Province, north western Iran. METHODS Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chlorof...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۷، شماره ۴، صفحات ۸-۱۶
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